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Millipore/AB15894 | Anti-Tbr2 Antibody/AB15894/100 µg
  • Millipore/AB15894 | Anti-Tbr2 Antibody/AB15894/100 µg

Millipore/AB15894 | Anti-Tbr2 Antibody/AB15894/100 µg

價格: ¥4308.00 市場價: 7180.00

貨號: AB15894
品牌: Millipore
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    • Description
      CatalogueNumberAB15894
      DescriptionAnti-Tbr2Antibody
      AlternateNames
      • Eomesoderminhomolog
      • T-boxbrainprotein2
      • T-brain-2
      • TBR-2
      • Tbr2
      BackgroundInformationEomesoderminhomolog(UniProtO54839;alsoknownasT-boxbrainprotein2,T-brain-2,TBR-2)isencodedbytheEomes(alsoknownasTbr2,C77258)gene(GeneID13813)inmurinespecies.Tbr2isamemberofaconservedproteinfamilythatsharesacommonDNA-bindingdomain,theT-box.T-boxgenesencodetranscriptionfactorsinvolvedintheregulationofdevelopmentalprocesses.Trb2hasbeenshowntoplayaroleinthedevelopmentoftheneocortexandfunctionsinthedifferentiationpathwayofrADIalgliatopost-mitoticprojectionneuron.
      ProductInformation
      FormatAffinityPurified
      Control
      • E13-14mousebrainlysate
      PresentationPurifiedchickenpolyclonalantibodyinbuffercontaining0.1MTris-Glycine(pH7.4),150mMNaClwith0.05%sodiumazide.
      StorageandShippingInformation
      StorageConditionsStablefor1yearat2-8°Cfromdateofreceipt.
      Applications
      ApplicationThisAnti-Tbr2antibodyisvalidatedforuseinWesternBlottingandImmunohistochemistry(Paraffin)forthedetectionofTbr2.
      KeyApplications
      • WesternBlotting
      • Immunohistochemistry
      ApplicationNotesImmunohistochemistryAnalysis:A1:1,000dilutionfromarepresentativelotdetectedTbr2inmousecerebralcortexandmousecerebellumtissue.
      WesternBlot(SNAPID)Analysis:0.5μg/mlfromapreviouslotdetectedTbr2in10μgofE13-14mousebrainlysate.
      ImmunohistochemistryAnalysis:1:100dilutionfromapreviouslotdetectedTbr2inmouseandhumanbrain.
      BIOLOGicalInformation
      ImmunogenKLH-conjugatedlinearpeptidecorrespondingtomouseTbr2.
      EpitopeTranscriptionActivationDomain
      ConcentrationPleaserefertotheCertificateofAnalysisforthelot-specificconcentration.
      HostChicken
      SpecificityExpectedtoreactwithbothsplicedisoformsofmurineTbr2.
      IsotypeIgY
      SpeciesReactivity
      • Mouse
      • Human
      • Rat
      AntibodyTypePolyclonalAntibody
      EntrezGeneNumber
      GeneSymbol
      • Eomes
      • Tbr2
      PurificationMethodAffinityPurfied
      UniProtNumber
      UniProtSummaryFUNCTION:Functionsasatranscriptionalactivatorplayingacrucialroleduringdevelopment.Functionsintrophoblastdifferentiationandlateringastrulation,regulatingbothmesodermdelaminationandendodermspecification.PlaysaroleinbraindevelopmentbeingrequiredforthespecificationandtheproliferationoftheintermediateProgenitorcellsandtheirprogenyinthecerebralcortex.AlsoinvolvedinthedifferentiationofCD8+T-cellsduringimmuneresponseregulatingtheexpressionoflyticeffectorgenes.

      SUBCELLULARLOCATION:NucleusProbable.

      TISSUESPECIFICTY:ExpressedinCD8+T-cells.

      DEVELOPMENTALSTAGE:Originallyexpressedinthetrophoectodermoftheblastocystandlaterintheextra-embryonicectodermoftheearlypost-implantationembryo.Intheembryoproper,expressedintheposteriorpartoftheepIBLast.Duringgastrulation,extendsdistallyintotheprimitivestreakandnascentmesoderm.Alsoexpressedinthedevelopingforebrainandtheolfactorylobes.ExpressedatE12.5andE14.5intheforebrain.

      INDUCTION:Up-regulatedinCD8+T-cellsuponactivation.

      DISRUPTIONPHENOTYPE:Embryoniclethalduetoperi-implantationdefects.Mutantembryosarrestsoonafterimplantationandfailtoformorganizedembryonicorextraembryonicstructures.Conditionalmutants,withexpressionabrogatedintheinnercellmassofembryosfromearlyimplantationstagesonward,displaygastrulationdefects.

      SEQUENCESIMLARITIES:Contains1T-boxDNA-bindingdomain.
      MolecularWeight~85/95kDaobserved.Targetbandsappearlargerthanthecalculatedmolecularsizeof74.8kDa(isoform1)and72.7kDa(isoform2).
      PhysicochemicalInformation
      Dimensions
      MaterialsInformation
      MaterialsInformation
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