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Millipore/17-497 | Ras Activation ELISA ASSAY Kit/17-497/96 assays
  • Millipore/17-497 | Ras Activation ELISA ASSAY Kit/17-497/96 assays

Millipore/17-497 | Ras Activation ELISA ASSAY Kit/17-497/96 assays

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貨號(hào): 17-497
品牌: Millipore
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    • Description
      CatalogueNumber17-497
      Replaces17-424
      BrandFamilyUpstate
      TradeName
      • Upstate
      DescriptionRasActivationELISAASSAYKit
      BackgroundInformationRasproteinsfunctionasGDP/GTP-regulatedbinaryswitchesinsignaltransductioncascadesthatcanleadtocellgrowth,proliferation,differentiation,orsurvival.ThisRassuperfamilyhasatleastfivemajorbranchesthatincludeRas,Rho,Ran,Arf/Sar,Rab.Rasitselfhas3primaryisoforms(H-Ras,N-Ras,andK-Ras)thatdifferintheirapproximately20C-terminalaminoacids.TheyarecollectivelyreferredtoasRas.Initsactiveform,RasisboundtoGTP.Thiscausesaconformationalchangethatallowittointeractandbindtoseveraleffectormolecules,mostnotablythemembersoftheRaffamily,theRalGDSfamily,andPhosphoinositide3-kinases(PI3Kinase).RasthencleavesGTPtoGDPresultinginitsinactivation.Initsoncogenic,mutatedstate,RasisunabletohydrolyzeGTPtoGDP,thusstayinginanactivestateandactivatingnumerouspathways.
      Duetoitsroleasakeyregulatorofcellularfunctionsanditsimplicationsinvariouscancers,Rashasbeenapopulartargetforcancerresearchandanti-cancertherapeuticsforthepasttwodecadesforbothacademicandpharmaceuticalresearch.Itwasalsothefirsthumanoncogeneidentified.MutationsincellularRashavebeenfoundtobepresentinalargepercentageofallhumancancers.Morespecifically,K-Rasmutationsoccurfrequentlyinlung,pancreatic,andcoloncancers,whereasH-Rasmutationsareprevalentinbladder,kidney,andthyroidcancers,andN-Rasmutationsareassociatedwithmelanoma,hepatocellularcarcinoma,andleukemia(3).Inrecentstudies,RasactivityhasalsobeenimplicatedinanumberofhumandevelopmentdefectssuchasCostellosyndromeandNoonansyndrome(4).
      OnepaththatthepharmaceuticalindustryhastakentocontrolRasanditsactivityisbyattackingwhatsomeconsideritsAchilles’heel.Foritsactivation,Rasmustlocalizetotheplasmamembrane,butinterestingly,itlacksatransmembranedomain.Toachievethis,Rasmustfirstundergoapost-translationalmodificationknownasprenylationorgeranylationatitsC-terminalCAAXmotif.ThesedrugshaveyettopassclinicaltrialsthoughandthereisdoubtthattheywilleverbesuccessfulintreatingtumorsassociatedwithRasactivation.
      MaterialsRequiredbutNotDelivered1.Multi-channelorrepeatingPipettes
      2.Plateshakeratroomtemperatureandat4oC.
      3.Pipettors&tipscapableofaccuratelymeasuring1-1000μL
      4.Graphingsoftwareforplottingdataorgraphpaperformanualplottingofdata
      5.Multi-channelpipettorreservoirs
      6.Proteaseinhibitors
      7.Nucleasefreewater/deionizedwater
      8.PBS
      9.LuminometerorCCDcameracoupledimagingsystem
      ProductInformation
      Components
      • 1.Glutathione-CoatedPlate,96-wellstripformat:(PartNo.3007063)1platecontaining12x8strip-wellscoatedwithglutathione.
      • 2.Anti-RasDetectionAntibody:(PartNo.2006992)Onevialcontaining10μLofanti-Rasmousemonoclonalprimaryantibody.CanspecificallydetectK-,H-,N-isoformsofRas.
      • 3.Mg2+Lysis/WashBuffer,5X:(PartNo.CS202617)1vialcontaining18mLof5XMg2+Lysis/WashBufferforpreparationofcelllysate.
      • 4.20%Tween?20:(PartNo.#20-246)Onevialcontaining3mLof20%Tween?20.
      • 5.10%BSAinTBS(BlockingBuffer):(PartNo.20-191)Onebottlecontaining10mL10%BSAin1XTBS.
      • 6.20XTBSWash:(PartNo.20-190)Onebottlecontaining50mLof20XTBS,pH7.4.
      • 7.GtxMs,HRP:(PartNo.CS202619)Oneambervialcontaining25μLofgoatanti-mouseHRPconjugatedantibody.
      • 8.ChemiluminescentReactionBuffer:(PartNo.90496)Oneamberbottlecontaining8mLofChemiluminescentReactionBuffer.Keepoutoflight.
      • 9.ChemiluminescentDetectionReagent:(PartNo.90495)Onebottlevialcontaining4mLofChemiluminescentDetectionReagent.Keepoutoflight.
      • Storeat-80oC
      • 10.Raf-1-RBD,GST:(PartNo.14-863)2vialscontaining150μLofRaf-1-RBDat2μg/μL.
      • 11.EGFstimulatedHeLaCellLysate:(PartNo.12-500)2vialscontaining40μLoflysateat5mg/mLconcentration,lysedinMg2+Lysis/Washbuffer(Catalog#CS202617).
      StorageandShippingInformation
      Applications
      KeyApplications
      • ELISA
      • CellFunctionAssay
      BIOLOGicalInformation
      SpeciesReactivity
      • Human
      • Mouse
      • Rat
      AnalytesAvailable
      • Ras
      EntrezGeneNumber
      EntrezGeneSummaryK-Ras
      Thisgene,aKirstenrasoncogenehomologfromthemammalianrasgenefamily,encodesaproteinthatisamemberofthesmallGTPasesuperfamily.AsingleaminoacidsubstitutionisresponsIBLeforanactivatingmutation.Thetransformingproteinthatresultsisimplicatedinvariousmalignancies,includinglungadenocarcinoma,mucinousadenoma,ductalcarcinomaofthepancreasandcolorectalcarcinoma.AlternativesplicingleadstovariantsencodingtwoisoformsthatdifferintheC-terminalregion.[providedbyRefSeq]
      H-Ras
      ThisgenebelongstotheRasoncogenefamily,whosemembersarerelatedtothetransforminggenesofmammaliansarcomaretroviruses.Theproductsencodedbythesegenesfunctioninsignaltransductionpathways.TheseproteinscanbindGTPandGDP,andtheyhaveintrinsicGTPaseactivity.Thisproteinundergoesacontinuouscycleofde-andre-palmitoylation,whichregulatesitsrapidexchangebetweentheplasmamembraneandtheGolgiapparatus.MutationsinthisgenecauseCostellosyndrome,adiseasecharacterizedbyincreasedgrowthattheprenatalstage,growthdeficiencyatthepostnatalstage,predispositiontotumorformation,mentalretardation,skinandmusculoskeletalabnormalities,distinctivefacialappearanceandcardiovascularabnormalities.Defectsinthisgeneareimplicatedinavarietyofcancers,includingbladdercancer,follicularthyroidcancer,andoralsquamouscellcarcinoma.Multipletranscriptvariants,whichencodedifferentisoforms,havebeenidentifiedforthisgene.[providedbyRefSeq]
      GeneSymbol
      • KRAS
      • K-Ras
      • v-Ki-ras2Kirstenratsarcomaviraloncogenehomolog
      • K-rasp21protein
      • HRAS
      • H-Ras
      • Ha-Ras
      • NRAS
      • N-Ras
      • neuroblastomaRASviral(v-ras)oncogenehomolog
      UniProtNumber
      PhysicochemicalInformation
      Dimensions
      MaterialsInformation
      MaterialsInformation
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