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Millipore/17-10121 | ChIPAb+ Acetyl-Histone H4 (Lys12) - ChIP Validated Antibody and Primer Set, rabbit monoclonal/17-10
  • Millipore/17-10121 | ChIPAb+ Acetyl-Histone H4 (Lys12) - ChIP Validated Antibody and Primer Set, rabbit monoclonal/17-10

Millipore/17-10121 | ChIPAb+ Acetyl-Histone H4 (Lys12) - ChIP Validated Antibody and Primer Set, rabbit monoclonal/17-10

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貨號: 17-10121
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    • Description
      CatalogueNumber17-10121
      TradeName
      • ChIPAb+
      • Upstate
      DescriptionChIPAb+Acetyl-HistoneH4(Lys12)-ChIPValidatedAntibodyandPrimerSet,rabbitmonoclonal
      OverviewAllChIPAb+antibodiesareindividuallyvalidatedforchromatinprecipitation,everylot,everytime.EachChIPAb+antibodysetincludescontrolprimers(testedeverylotbyqPCR)toBIOLOGicallyvalidateyourIPresultsinalocus-specificcontext.TheqPCRprotocolandprimersequencesareprovided,allowingresearcherstovalidateChIPprotocolswhenusingourantibodyintheirchromatincontext.EachsetalsoincludesanegativecontrolantibodytoensurespecificityoftheChIPreaction.
      TheChIPAb+Acetyl-HistoneH4(Lys12)setincludestheAcetyl-HistoneH4(Lys12)antibody,anegativecontrolnormalrabbitIgG,andqPCRprimerswhichamplifya213bpregionofhumanGAPDHcodingregion.TheAcetyl-HistoneH4(Lys12)andnegativecontrolsaresuppliedinascalable"perChIP"reactionsizeandcanbeusedtofunctionallyvalidatetheprecipitationofAcetyl-HistoneH4(Lys12)associatedchromatin.
      AlternateNames
      • H4K12Ac
      • HistoneH4(acetylK12)
      ProductInformation
      FormatPurified
      Control
      • IncludesnegativecontrolsupernatantandprimersspecificforhumanGAPDHcodingregion.
      PresentationAnti-Acetyl-HistoneH4(Lys12)(rabbitmonoclonal),.Onevialcontaining50μLofrabbitmonoclonalIgGcellculturesupernatantin0.1%sodiumazide.Storeat-20°C.
      NegativeControlSupernatant,.Onevialcontaining100μLofculturedsupernatantin0.05%sodiumazide.Storeat-20°C.
      ChIPPrimers,humanGAPDHcodingregion,.Onevialcontaining75μLof5μMeachprimerspecificforhumanGAPDHcodingregion.
      Storeat-20°C.
      FOR:GGCTCCCACCTTTCTCATCC
      REV:GGCCATCCACAGTCTTCTGG
      StorageandShippingInformation
      StorageConditionsStablefor1yearat-20°Cfromdateofreceipt.HandlingRecommendations:Uponfirstthaw,andpriortoremovingthecap,centrifugethevialandgentlymixthesolution.Aliquotintomicrocentrifugetubesandstoreat-20°C.Avoidrepeatedfreeze/thawcycles,whichmaydamageIgGandaffectproductperformance.
      Applications
      ApplicationThisChIPAb+Acetyl-HistoneH4(Lys12)-ChIPValidatedAntibody&PrimerSetconvenientlyincludestheantibody&thespecificcontrolPCRprimers.
      KeyApplications
      • WesternBlotting
      • CellFunctionAssay
      • ChromatinImmunoprecipitation(ChIP)
      ApplicationNotesChromatinImmunoprecipitation:
      SonicatedchromatinpreparedfromHeLacells(1X10E6cellequivalentsperIP)weresubjectedtochromatinimmunoprecipitationusingeither2μLofNegativeControlSupernatant,or2μLofAnti-Acetyl-HistoneH4(Lys12)andtheMagnaChIP?AKit(Cat.#17-610).
      SuccessfulimmunoprecipitationofAcetyl-HistoneH4(Lys12)-associatedDNAfragmentswasverifiedbyqPCRusingChIPPrimers,humanGAPDHCodingRegionasapositivelocus,andagenedesertregionasanegativelocus.(Figure2).DataispresentedaspercentinputofeachIPsamplerelativetoinputchromatinforeachampliconandChIPsampleasindicated.
      PleaserefertotheEZ-MagnaChIP?A(Cat.#17-408)orEZ-ChIP?(Cat.#17-371)protocolforexperimentaldetails.
      WesternBlotAnalysis:
      Representativelotdata.
      LysatesfromHeLacellsuntreatedorsodiumbutyratetreated(Lanes1and2respectively)wereresolvedprobedwithanti-acetyl-HistoneH4(Lys12)(1:1,000).ArrowindicatesAcetyl-HistoneH4(Lys12).
      ArrowindicatesAcetyl-HistoneH4(Lys12)(~11kDa)(Figure3).
      BiologicalInformation
      ImmunogenPeptidecorrespondingtoHistoneH4containingthesequence[GLG-AcK-GGA]onwhichLys12isacetylated.
      EpitopeUnknown
      HostRabbit
      SpecificityThisantibodydetectshistoneH4acetylatedatLys12.
      IsotypeIgG
      SpeciesReactivity
      • Human
      • Vertebrates
      SpeciesReactivityNoteReactswithHuman,widerangeofcross-reactivityexpectedbasedonsequencehomology.
      AntibodyTypeMonoclonalAntibody
      EntrezGeneNumber
      EntrezGeneSummaryHistonesarebasicnuclearproteinsthatareresponsIBLeforthenucleosomestructureofthechromosomalfiberineukaryotes.Twomoleculesofeachofthefourcorehistones(H2A,H2B,H3,andH4)formanoctamer,aroundwhichapproximately146bpofDNAiswrappedinrepeatingunits,callednucleosomes.Thelinkerhistone,H1,interactswithlinkerDNAbetweennucleosomesandfunctionsinthecompactionofchromatinintohigherorderstructures.ThisgeneisintronlessandencodesamemberofthehistoneH4family.TranscriptsfromthisgenelackpolyAtailsbutinsteadcontainapalindromicterminationelement.Thisgeneisfoundinthelargehistonegeneclusteronchromosome6.
      GeneSymbol
      • HIST1H4A
      • H4/J
      • HIST1H4F
      • HIST1H4L
      • H4FN
      • H4FA
      • H4FH
      • HIST1H4H
      • H4F2
      • H4FM
      • H4/A
      • H4FK
      • H4FG
      • HIST2H4
      • H4FB
      • H4/K
      • HIST1H4I
      • H4/N
      • HIST1H4B
      • H4FE
      • H4/M
      • H4/a
      • HIST1H4E
      • HIST4H4
      • HIST2H4A
      • H4FC
      • H4FI
      • H4/H
      • HIST1H4C
      • H4/G
      • HIST1H4K
      • H4FJ
      • H4FD
      • H4/I
      • H4/B
      • H4/D
      • H4/C
      • HIST1H4J
      • HIST1H4D
      • H4/E
      Modifications
      • Acetylation
      PurificationMethodProteinAPurfied
      UniProtNumber
      UniProtSummaryFUNCTION:SwissProt:P62805#Corecomponentofnucleosome.NucleosomeswrapandcompactDNAintochromatin,limitingDNAaccessibilitytothecellularmachinerieswhichrequireDNAasatemplate.Histonestherebyplayacentralroleintranscriptionregulation,DNArepair,DNAreplicationandchromosomalstABIlity.DNAaccessibilityisregulatedviaacomplexsetofpost-translationalmodificationsofhistones,alsocalledhistonecode,andnucleosomeremodeling.
      SIZE:103aminoacids;11367Da
      SUBUNIT:ThenucleosomeisahistoneoctamercontainingtwomoleculeseachofH2A,H2B,H3andH4assembledinoneH3-H4heterotetramerandtwoH2A-H2Bheterodimers.Theoctamerwrapsapproximately147bpofDNA.
      SUBCELLULARLOCATION:Nucleus.
      PTM:SymmetricdimethylationonArg-4bythePRDM1/PRMT5complexmayplayacrucialroleinthegerm-celllineage(Bysimilarity).&UbiquitinatedbytheCUL4-DDB-RBX1complexinresponsetoultravioletirrADIation.ThismayweakentheinteractionbetweenhistonesandDNAandfacilitateDNAaccessibilitytorepairproteins.
      SIMILARITY:SwissProt:P62805##BelongstothehistoneH4family.
      MolecularWeight11kDa
      PhysicochemicalInformation
      Dimensions
      MaterialsInformation
      MaterialsInformation
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