當(dāng)前位置 ： Millipore >>> Millipore/17-10099 | ChIPAb+ Acetyl-Histone H4 (Lys8) - ChIP Validated Antibody and Primer Set/17-10099/25 assays25 assa
Millipore/17-10099 | ChIPAb+ Acetyl-Histone H4 (Lys8) - ChIP Validated Antibody and Primer Set/17-10099/25 assays25 assa
  • Millipore/17-10099 | ChIPAb+ Acetyl-Histone H4 (Lys8) - ChIP Validated Antibody and Primer Set/17-10099/25 assays25 assa

Millipore/17-10099 | ChIPAb+ Acetyl-Histone H4 (Lys8) - ChIP Validated Antibody and Primer Set/17-10099/25 assays25 assa

價(jià)格: ￥4800.00 市場(chǎng)價(jià): 8000.00
貨號(hào): 17-10099
品牌: Millipore
規(guī)格
數(shù)量
庫(kù)存(0)
特別 提示
代購(gòu)產(chǎn)品：無(wú)質(zhì)量問(wèn)題不接受退換貨，下單前請(qǐng)仔細(xì)核對(duì)信息。
下單后請(qǐng)及時(shí)聯(lián)系客服核對(duì)商品價(jià)格，訂單生效后再付款。
資深產(chǎn)品顧問(wèn)
咨詢顧問(wèn)

全國(guó)免費(fèi)服務(wù)熱線

4000-520-616

  • 自營(yíng)商城 一站式服務(wù)
  • 廠家直采 剔除溢價(jià)
  • 品質(zhì)甄選 正品保證
  • 嚴(yán)控流程 只做188精品
  • 極速物流 如約送貨
  • 詳情
  • 使用說(shuō)明
  • 常見(jiàn)問(wèn)題
    • Description
      CatalogueNumber17-10099
      BrandFamilyUpstate
      TradeName
      • ChIPAb+
      • Upstate
      DescriptionChIPAb+Acetyl-HistoneH4(Lys8)-ChIPValidatedAntibodyandPrimerSet
      OverviewAllChIPAb+antibodiesareindividuallyvalidatedforchromatinprecipitation,everylot,everytime.EachChIPAb+antibodysetincludescontrolprimers(testedeverylotbyqPCR)toBIOLOGicallyvalidateyourIPresultsinalocus-specificcontext.TheqPCRprotocolandprimersequencesareprovided,allowingresearcherstovalidateChIPprotocolswhenusingourantibodyintheirchromatincontext.EachsetalsoincludesanegativecontrolantibodytoensurespecificityoftheChIPreaction.
      TheChIPAb+Acetyl-HistoneH4(Lys8)setincludestheAcetyl-HistoneH4(Lys8)antibody,anegativecontrolrabbitserum,andqPCRprimerswhichamplifya134bpregionofhumanHSPCA.TheAcetyl-HistoneH4(Lys8)andnegativecontrolsaresuppliedinascalable"perChIP"reactionsizeandcanbeusedtofunctionallyvalidatetheprecipitationofAcetyl-HistoneH4(Lys8)-associatedchromatin.
      AlternateNames
      • H4K8Ac
      • HistoneH4(acetylK8)
      BackgroundInformationHistoneH4isoneofthe5mainhistoneproteinsinvolvedinthestructureofchromatinineukaryoticcells.FeaturingamainglobulardomainandalongNterminaltailH4isinvolvedwiththestructureofthenucleosomesofthe"beadsonastring"structure.

      TheN-terminaltailofhistoneH4protrudesfromtheglobularnucleosomecoreandcanundergoseveraldifferenttypesofepigeneticmodificationsthatinfluencecellularprocesses.Thesemodificationsincludethecovalentattachmentofmethyloracetylgroupstolysineandarginineaminoacidsandthephosphorylationofserineorthreonine.
      ProductInformation
      FormatSerum
      Control
      • IncludesnegativecontrolnormalrabbitserumantibodyandprimersspecificforhumanHSPCA.
      PresentationAnti-Acetyl-HistoneH4(Lys8)(rabbitpolyclonal).Onevialcontaining50μLofantiserumcontaining0.05%sodiumazide.Storeat-20°C.

      NormalRabbitSerum.Onevialcontaining100μLofantiserumcontaining0.05%sodiumazide.
      Storeat-20°C.

      ChIPPrimers,HSPCA.Onevialcontaining75μLof5μMofeachprimerspecificforhumanHSPCA.
      Storeat-20°C.
      FOR:GCAACAGCTACCACAGGACCA
      REV:GAGCGTGTGAAATCAACATAAAGC
      StorageandShippingInformation
      StorageConditionsStablefor1yearat-20°Cfromdateofreceipt.HandlingRecommendations:Uponfirstthaw,andpriortoremovingthecap,centrifugethevialandgentlymixthesolution.Aliquotintomicrocentrifugetubesandstoreat-20°C.Avoidrepeatedfreeze/thawcycles,whichmaydamageIgGandaffectproductperformance.
      Applications
      ApplicationTheChIPAb+Acetyl-HistoneH4(Lys8)setincludestheAcetyl-HistoneH4(Lys8)antibody,anegativecontrolrabbitserum&qPCRprimerswhichamplifya134bpregionofhumanHSPCA.
      KeyApplications
      • WesternBlotting
      • ChromatinImmunoprecipitation(ChIP)
      ApplicationNotesChromatinImmunoprecipitation:
      SonicatedchromatinpreparedfromHeLacells(1X106cellequivalentsperIP)weresubjectedtochromatinimmunoprecipitationusingeither2μLofNormalRabbitSerumor2μLofAnti-Acetyl-HistoneH4(Lys8)andtheMagnaChIP?AKit(Cat.#17-610).
      SuccessfulimmunoprecipitationofAcetyl-HistoneH4(Lys8)associatedDNAfragmentswasverifiedbyqPCRusingChIPPrimers,humanHSPCAforapositive,andknownNegativePrimers(Martinato,F.,etal.,2008)asanegativeassay(Pleaseseefigures).
      DataispresentedaspercentinputofeachIPsamplerelativetoinputchromatinforeachampliconandChIPsampleasindicated.
      PleaserefertotheEZ-MagnaChIP?A(Cat.#17-408)orEZ-ChIP?(Cat.#17-371)protocolforexperimentaldetails.

      WesternBlotAnalysis:
      Representativelotdata.
      SodiumButyrate-treatedHeLaacidextractwasresolvedbyelectrophoresis,transferredtoPVDFmembraneandprobedwithAnti-AcetylHistoneH4(Lys8)(1:1000dilution).Todemonstratespecificity,serumwaspreincubatedwithmodifiedHistoneH4peptides:
      Lane1:Nopeptide
      Lane2:acetyl-HistoneH4(K5)(Cat.#:12-343)
      Lane3:acetyl-HistoneH4(K8)(Cat.#:12-344)
      Lane4:acetyl-HistoneH4(K12)(Cat.#:12-345)
      Lane5:acetyl-HistoneH4(K16)(Cat.#:12-346)
      Lane6:acetyl-HistoneH4(K5,8,12,16)(Cat.#:12-353)
      Lane7:HistoneH4(Cat.#:12-347)
      Proteinswerevisualizedusingadonkeyanti-rabbitIgGconjugatedtoHRPandvisualizedusingachemiluminescencedetectionsystem(Pleaseseefigures).

      Immunoprecipitation:5μLofapreviouslotimmunoprecipitatedacetylHistoneH4from1mgofwildtypewholecellyeastlysatesbutnotfromayeaststraincontainingaHistoneH4Lys8Rsubstitution.
      BiologicalInformation
      ImmunogenSyntheticpeptidecorrespondingtoyeastHistoneH4acetylatedatlysine8.
      HostRabbit
      SpecificityRecognizesacetyl-HistoneH4(Lys8).
      SpeciesReactivity
      • Human
      • Yeast(S.cerevisiae)
      SpeciesReactivityNoteDemonstratedtoreactwithYeastandhuman.Broadspeciescross-reactivityisexpectedbasedon100%sequencehomology.
      AntibodyTypePolyclonalAntibody
      EntrezGeneNumber
      EntrezGeneSummaryHistonesarebasicnuclearproteinsthatareresponsIBLeforthenucleosomestructureofthechromosomalfiberineukaryotes.Twomoleculesofeachofthefourcorehistones(H2A,H2B,H3,andH4)formanoctamer,aroundwhichapproximately146bpofDNAiswrappedinrepeatingunits,callednucleosomes.Thelinkerhistone,H1,interactswithlinkerDNAbetweennucleosomesandfunctionsinthecompactionofchromatinintohigherorderstructures.ThisgeneisintronlessandencodesamemberofthehistoneH4family.TranscriptsfromthisgenelackpolyAtailsbutinsteadcontainapalindromicterminationelement.Thisgeneisfoundinthelargehistonegeneclusteronchromosome6.
      GeneSymbol
      • HIST1H4A
      • H4/J
      • HIST1H4F
      • HIST1H4L
      • H4FN
      • H4FA
      • H4FH
      • HIST1H4H
      • H4F2
      • H4FM
      • H4/A
      • H4FK
      • H4FG
      • HIST2H4
      • H4FB
      • H4/K
      • HIST1H4I
      • H4/N
      • HIST1H4B
      • H4FE
      • H4/M
      • H4/a
      • HIST1H4E
      • HIST4H4
      • HIST2H4A
      • H4FC
      • H4FI
      • H4/H
      • HIST1H4C
      • H4/G
      • HIST1H4K
      • H4FJ
      • H4FD
      • H4/I
      • H4/B
      • H4/D
      • H4/C
      • HIST1H4J
      • HIST1H4D
      • H4/E
      Modifications
      • Acetylation
      PurificationMethodUnpurified
      UniProtNumber
      UniProtSummaryFUNCTION:SwissProt:P62805#Corecomponentofnucleosome.NucleosomeswrapandcompactDNAintochromatin,limitingDNAaccessibilitytothecellularmachinerieswhichrequireDNAasatemplate.Histonestherebyplayacentralroleintranscriptionregulation,DNArepair,DNAreplicationandchromosomalstABIlity.DNAaccessibilityisregulatedviaacomplexsetofpost-translationalmodificationsofhistones,alsocalledhistonecode,andnucleosomeremodeling.
      SIZE:103aminoacids;11367Da
      SUBUNIT:ThenucleosomeisahistoneoctamercontainingtwomoleculeseachofH2A,H2B,H3andH4assembledinoneH3-H4heterotetramerandtwoH2A-H2Bheterodimers.Theoctamerwrapsapproximately147bpofDNA.
      SUBCELLULARLOCATION:Nucleus.
      PTM:SymmetricdimethylationonArg-4bythePRDM1/PRMT5complexmayplayacrucialroleinthegerm-celllineage(Bysimilarity).&UbiquitinatedbytheCUL4-DDB-RBX1complexinresponsetoultravioletirrADIation.ThismayweakentheinteractionbetweenhistonesandDNAandfacilitateDNAaccessibilitytorepairproteins.
      SIMILARITY:SwissProt:P62805##BelongstothehistoneH4family.
      MolecularWeight~11kDa
      PhysicochemicalInformation
      Dimensions
      MaterialsInformation
      MaterialsInformation
    售后保障
    螞蟻淘生物188，秉承螞蟻淘一貫的嚴(yán)謹(jǐn)態(tài)度，由螞蟻淘公司專業(yè)人員負(fù)責(zé)品控、采購(gòu)、物流、銷售、售后，保障正品優(yōu)質(zhì)。以“快速好省，為科研提供好產(chǎn)品、好價(jià)格”為理念，直接鏈接原廠家，從全國(guó)各地原制造商嚴(yán)格挑選188款科研精品，剔除品牌溢價(jià)，188生物新電商，把好的產(chǎn)品帶給科研！? 力求給你最優(yōu)質(zhì)的商品。
  • Q：生物188產(chǎn)品正品保障嗎？
    A：生物188質(zhì)量把控人員具有十年的從業(yè)經(jīng)驗(yàn)，在業(yè)界享有良好的口碑；自營(yíng)商城，直接從廠家采購(gòu)， 自己的團(tuán)隊(duì)負(fù)責(zé)國(guó)際物流和清關(guān)，中間沒(méi)有第三方，所有流程嚴(yán)格把控，100%保證正品，假一罰十。

    Q：下單后可以修改訂單嗎？
    A：下單后的商品付款之前可以修改；訂單付款成功，需要聯(lián)系我們客服進(jìn)行修改；客服電話：4000-520-616

    Q：可以開(kāi)發(fā)票嗎？
    A：本網(wǎng)站所售商品都是正規(guī)清關(guān)，均開(kāi)具16%正規(guī)發(fā)票，發(fā)票金額含配送費(fèi)金額，另有說(shuō)明的除外。

    Q：商品幾天可以發(fā)貨？
    A：生物188商品，全部現(xiàn)貨銷售，付款后即可發(fā)貨,一般一周內(nèi)送達(dá)！

    Q：如何聯(lián)系商家？
    A：有任何問(wèn)題夠可以電話咨詢我們，全國(guó)24小時(shí)免費(fèi)服務(wù)熱線：4000-520-616 或聯(lián)系我們的在線客服QQ：1570468124

    Q：收到的商品少了/發(fā)錯(cuò)了怎么辦？
    A：同個(gè)訂單購(gòu)買多個(gè)商品可能會(huì)分為一個(gè)以上包裹發(fā)出，可能不會(huì)同時(shí)送達(dá)，建議查看訂單詳情是否是 部分發(fā)貨狀態(tài)；如未收到，可聯(lián)系在線客服或者致電4000-520-616。

    Q：退換貨/維修需要多長(zhǎng)時(shí)間？
    A：一般情況下，退貨處理周期為客戶收到產(chǎn)品一個(gè)月內(nèi)（以快遞公司顯示簽收時(shí)間為準(zhǔn)），包裝規(guī)格、 數(shù)量、品種不符，外觀毀損、短缺或缺陷，請(qǐng)?jiān)谑盏截?4小時(shí)內(nèi)申請(qǐng)退換貨；特殊商品以合同條款為準(zhǔn)。

何為188

極簡(jiǎn)而嚴(yán)謹(jǐn)，我們僅銷售188款生物醫(yī)學(xué)科研用品，款款都是爆款；因?yàn)樯偎跃劢梗劢拐邕x每一款產(chǎn)品，聚焦服務(wù)每一位客戶!

關(guān)注我們 :

點(diǎn)擊QQ聯(lián)系我們
生物188微信

關(guān)注188微信公眾號(hào)

獲取最新優(yōu)惠活動(dòng)通知