Application | Anti-PhosphotyrosineAntibody,clone4G10?,BiotinConjugatedetectstyrosinephosphorylatedproteinsinallspecies.ThisuniquemonoclonalantibodyispublishedinpeerreviewedjournalsandvalidatedforuseinIC,IP,WB. |
KeyApplications | - Immunocytochemistry
- Immunoprecipitation
- WesternBlotting
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ApplicationNotes | Immunoprecipitation: 4μgofapreviouslot,usedinconjunctionwithStreptavidin,agaroseconjugate(Catalog#16-126),immunoprecipitatedphosphotyrosinecontainingproteinsfromalysateofEGFstimulatedA431cells. Note:Topreservephosphotyrosine,add0.2mMsodiumorthovanadatetothelysisbuffer.
Immunocytochemistry: 5-10μg/mLofapreviouslotgavepositiveimmunostainingofEGF-stimulatedA431cellsthathadbeenfixedwithethanol:aceticacid[1:1].
ApplicationNotes Foruseinapplicationsinwhichabiotinconjugateisadvantageous,suchasWBandIC. |