Description
Dynabeads®M-450Tosylactivatedare4.5µmsuperparamagneticbeadscontainingsurfacetosylgroups.Thebeadscovalentlybindprimaryaminoandsulfhydrylgroupsinantibodiestopositionthemintheoptimalorientationforcell-surfaceproteinbinding.Lowbackgroundandcovalentbindingoftheantibodiesmakethesebeadsanexcellentchoiceforcellseparationapplicationswhenadownstreamreleasefromthebeadsisnotrequired.
•Quicklydepletecellsorpositivelyisolatetargetcellsfordownstreammolecularanalysis
•Excellentforcellisolationproceduresusingyourownuniqueantibody
•Severalantibodiescanbecoupledonthesamebeadsfornon-cell-basedapplications,suchascellactivation
CouplingprocedureCovalentcouplingisperformedovernightbyincubatingtheantibodyofchoicewiththeDynabeads®M-450Tosylactivatedbeads.OptimalcouplingoccursathighpH(8.5–9.5)andat37°C.ForpH-l
ABIleantibodies,couplingcanbeperformedinanalternativebufferatpH7.4.
Notrecommendedforimmunoprecipitation(IP)These4.5µmTosylactivatedbeadsarenotrecommendedforIPastheyhavealowersurfacecapacityperunitmasswhencomparedto1µmand2.8µmTosylactivatedandEpoxyDynabeads®.
LearnmoreaboutDynabeads®products•FindDynabeads®productsforawholerangeofapplications.
•FindmagnetsforDynabeads®separations.
ForResearchUseOnly.Notforuseindiagnosticprocedures.