Content:2mgOverview
Stem
MACS?CHIR99021isaselectivesmallmoleculeGSK3inhibitorthatactivatesWntsignaling.
Details
Backgroundinformation
StemMACS?CHIR99021isahighlyselectiveinhibitorofglycogensynthasekinase3(GSK-3),acrucialregulatoroftheWntsignalingpathway.TheaminopyridineCHIR99021inhibitsbothGSK-3isoforms,GSK-3α(IC5010nM)andGSK-3β(IC506.7nM).UnlikeotherGSK-3inhibitors,itdoesnotcross-reactwithcyclin-dependentkinases(CDKs).ActivationofWntsignalingviaCHIR99021-mediatedGSK-3inhibitioniswidelyusedtomodulatepluripotentstemcelldifferentiationandself-renewal.
Applications
PublishedapplicationsforCHIR99021include:
- Na?ve,groundstatecultureofmurineESandiPScellsusing2iconditions1
- Na?ve,groundstatecultureofratESandiPScellsusing3iconditions2
- Na?ve,groundstatecultureofhumanESandiPScells3
- DifferentiationofhumanESandiPScellsintocardiomyocytes4
- DifferentiationofhumanESandiPScellsintoskeletalmuscle5
- DifferentiationofhumanESandiPScellsintodefinitiveendoderm6
- ChemicalReprogrammingofmousefibroblasts5