First,theLy-6B.2(7/4)
+cellsaremagneticallylabeledwithAnti-Ly-6B.2(7/4)MicroBeads.Then,thecells
USPensionisloadedontoa
MACS?Column,whichisplacedinthemagneticfieldofaMACSSeparator.ThemagneticallylabeledLy-6B.2(7/4)
+cellsareretainedwithinthecolumn.Theunlabeledcellsrunthrough;thiscellfractionisthusdepletedofLy-6B.2(7/4)
+cells.Afterremovingthecolumnfromthemagneticfield,theretainedLy-6B.2(7/4)
+cellscanbeelutedasthepositivelyselectedcellfraction.Toincreasethepurityofspleencells,thepositivelyselectedcellfractioncontainingtheLy-6B.2(7/4)
+cellsmustbeseparatedoverasecondcolumn.