Capacity:for1×109totalcellsOverview
TheCD146MicroBeadKitwasdevelopedfortheisolationofCD146+cells.ThekitconsistsofCD146MicroBeadsandFcRBlockingReagent.TheCD146MicroBeadsrecognizethehumanCD146antigen,alsoknownasMUC18,MCAM,Mel-CAM,andS-Endo-1.
Details
Backgroundinformation
CD146isatransmembraneglycoproteinbelongingtotheimmunoglobulinsuperfamilyandcontainsfiveextracellularimmunoglobulin(Ig)-likedomains.1,2
CD146possessesalimitedtissueexpressiondistribution,includingendothelialcells,smoothmusclecells,pericytes,folliculardendriticcells,melanomacells,andasubpopulationofactivatedTlymphocytes.1,3CD146+cellsisolatedfromumbilicalcord4,lipoaspirate5,dentalpulp,orendometrialtissue6,aredescribedtocontaincellswithclonogenicfibroblasticcolony(CFU-F)activityandmultilineagedifferentiationpotential,whichareoftencalledmesenchymalormarrowstromalcells(MSCs).
CD146functionsasaCa2+-independentcelladhesionmolecule,whichisinvolvedinheterophiliccell-cellinteractions7,andmaybeinvolvedintheextravasionand/orhomingofactivatedTcells3.CD146expressioninmelanomaisalsodirectlyassociatedwithtumorgrowthandmetastasis.7,8
Applications
TheCD146MicroBeadKitcanbeusedfor:
- isolationofhumanmesenchymalstromalcells(MSCs)fromlipoaspirateandothertissuesources,
- isolationofendothelialcellsandpericytesfromhumanendothelialtissue,
- isolationofhumanumbilicalveinendothelialcells(HUVECs)andhumanumbilicalcordperivascularcells(HUCPVCs)4fromumbilicalcordvein.
Columns
LSorXSColumns.