Capacity:for50preparationsOverview
TheNeuralTissueDissociationKitshavebeendevelopedforthegentleandefficientgenerationofsingle-cells
USPensionsfromneuraltissues.
Dependingontheapplicationthekitscanbeusedwithmanualmechanicaldissociationorwithautomateddissociationusingthegentle
MACS?Dissociators.
TheNeuralTissueDissociationKit-PostnatalNeuronshasbeenoptimizedforthespecialneedsofsensitivepostnatalneuronsduringdissociation.
Details
Backgroundinformation
Inlessthananhour,thegentletwo-stepenzymaticdissociationprocedureefficientlyyieldshighnumbersofviablecells.TheNeuralTissueDissociationKit(P)containspapain,andtheNeuralTissueDissociationKit(T)containstrypsin.
Someantigenepitopesaredamagedbypapain,othersbytrypsin.ThekitbasedonpapainisbestsuitedforusewithAnti-Prominin-1MicroBeads,CD11b(Microglia)MicroBeadsandAnti-A2B5MicroBeads,whereas,forexample,Anti-GLAST(ACSA-1)MicroBeadKitandAnti-PSA-NCAMMicroBeadsrequiretheuseofthetrypsin-basedkit.
Pleaseseefigure1forfurtherinformationwithregardtoepitopesensitivityandkitselection.