Capacity:for1×109totalcellsOverview
TheAnti-Sca-1MicroBeadKit(FITC)hasbeendevelopedforthepositiveselectionofhematopoieticstemand
Progenitorcellsfrommousebonemarrow.Sca-1
+stemcellshavebeenisolatedfromperipheralblood
1,fetal
2,andadultliver
3aswellasheart
4andprostatetissue
5.ThekitcanbeusedeitheraloneorincombinationwiththeLineageCellDepletionKit,mouseforenrichmentofKSLcellsaswellasincombinationwiththeCD105MultiSortKit(PE)fortheenrichmentoflong-termrepopulatinghematopoieticstemcells(LT-HSCs).
Details
Backgroundinformation
Sca-1(stemcellantigen-1)isoneofthedefining
MarkersforKSLcells(c-
kit
+Sca-1
+Lin
-),thepopulationthatcomprisesthestemcellcompartmentinmousebonemarrow.Sca-1expressionincombinationwithCD105canbefurtherusedtodefinelong-termrepopulatinghematopoieticstemcells(LTR-HSCs)
6,7.
TheSca-1proteinisencodedbyoneofthetwoLy-6A/Eallelesexpressedindifferentmousestrains(Ly-6E.1:e.g.BALB/c,C3H,NZB;Ly-6A.2:e.g.C57BL/6,SJL,129,AKR).Theanti-Sca-1monoclonalantibodyincludedintheSca-1MicroBeadKit(FITC)recognizesboth,Ly-6E.1andLy-6A.2.
Downstreamapplications
Bonemarrow-derivedSca-1
+cellshavebeenusedfornonhematopoieticdifferentiationintohepatocytesinvivo
8andneuralcellsinvitro
9.EScell-derivedSCA-1
+cellscouldbedifferentiatedintoendothelialcellswithhighpurityandyield
10.Fur
Thermore,smoothmusclecellshavebeengeneratedfromESCsviaisolationofSca-1
+cells
11.
Columns
MS,LS,XS,orauto
MACS?Columns.