Capacity:for2×109totalcellsOverview
TheCD146(LSEC)MicroBeadsweredevelopedforthepositiveselectionordepletionofCD146+cellsfrommousetissues.
Details
Backgroundinformation
CD146isatransmembraneglycoproteinandbelongstotheIgGsuperfamilyofcelladhesionmolecules.1TheCD146(LSEC)antibodybindstomouseliversinusoidalendothelialcells(LSECs)whicharemicrovascularendothelialcellsliningthehepaticsinusoidalwallandmainlycontributetothecontrolofimmuneresponsesagainstcirculatingsolubleantigensintheliver.Theirstrategicpositioningfavorsatightinteractionwithlymphocytesmigratingthroughtheliver.LSECspossessahighcapacityforantigenuptakeandprocessingbutexpress,incontrasttoprofessionalantigen-presentingcells(e.g.dendriticcells),onlylowlevelsofcostimulatorymolecules.2
TheCD146(LSEC)antibodyalsobindstoendothelialcellsfromavariousrangeoforgans,suchasskin,liver,kidney,brain,spleen,lymphnode,intestine,heartandskeletalmuscleaswellasonbloodvesselstructures,suchaspulmonaryarteries,veins,andthecapillarynetworkofthealveolarwallsbutnotonlymphaticendothelium.IncontrasttohumansthatexpressCD146alsoonTcellsorfolliculardendriticcells,murineCD146expressionwasonlyfoundonasubsetofNK1.1+cellsatlowlevels.3
Downstreamapplications
Endothelialcellsfromspleens,lungs,kidneys,andlivers3aswellasLSECsfromliver4havebeenselectedusingtheCD146(LSEC)MicroBeads.MouseLSECsisolatedwithCD146(LSEC)MicroBeadsaresuitableforfunctionalstudiesandco-cultureexperiments.
Columns
Forpositiveselection:MS,LS,XS,orauto
MACS?Columns.Fordepletion:LD,CS,D,orautoMACSColumns.