Arangeofapplicationsfromreversetranscription(RT)-PCRtomicroarrayanalysisand
CDNAlibrarygenerationrequirehigh-qualityRNA.UsingmRNAinsteadoftotalRNAasstartingmaterialcansignificantlyimprovethesensitivityandspecificityoftheresults.Thisisparticularlyimportant,whenthestartingmaterialislimitedorraretranscriptsareanalyzed.μMACSandMultiMACSmRNAIsolationKitsenableahighlysensitivemRNAisolation,fastereventhantr
ADItionaltotalRNAisolations.
Aftercelllysisandclearingofthecelllysate,themRNAismagneticallylabeledwithμMACSOligo(dT)MicroBeads.DuetothesmallsizeoftheMicroBeads,thehybridizationtothepoly(A)+tailofthemRNAmoleculesiscompletedwithinseconds.ThesampleisloadedontoaMACSColumnplacedinthemagneticfieldofaμMACSSeparator.Afterwashing,themagneticallylabeledmRNAisretainedonthecolumn.ByapplyingElutionBufferonthecolumn,puremRNAiselutedfromthecolumn.TheμMACSmRNAIsolationKitsaredesignedformanuallow-throughputmRNAisolationwhereastheMultiMACSmRNAIsolationKitsaredesignedforhigh-throughputapplicationsina96-wellformat.TheMultiMACSKitsareusedincombinationwiththeMultiMACSM96Separatorthatcanbeintegratedinaroboticsystemtoachieveafullyautomatedapproach.