Capacity:for1–3×109totalcellsOverview
TheCarcinomaCellEnrichmentKitwasdevelopedformorethana10,000-foldtheenrichmentofdisseminatedepithelialtumorcellsfromperipheralblood,bonemarrow,orlymphoidtissuebythepositiveselectionofcytokeratin7/8expressingcells.ForconvenientsubsequentimmunocytochemicaldetectiontheCarcinomaCellEnrichmentandDetectionKitcanbeused.
Details
Backgroundinformation
Mostmalignantcellswhichhavetheirorigininepithelialtissueexpresscytokeratinandcanberecognizedbyananti-cytokeratinantibody
1.WiththeCarcinomaCellEnrichmentKit,disseminatedepithelialtumorcellscanrapidlyandefficientlybeenrichedusing
MACSCytokeratinMicroBeads,e.g.fromafrequencyof1tumorcellin10
7leukocytestoafrequencyof1tumorcellper10
3leukocytes.Thus,largepatientsamplescanbenarrowedandscreenedfordisseminatedtumorcellsononlyafewmicroscopyslides.Detectionratesofocculttumorcellscanbeincreasedtomorethan80%
1,2.
Detailedseparationprocedure
TumorcellsofepithelialoriginsuchasmetastaticcarcinomasexpresscytokeratinsandcanbepositivelyselectedbyusingAnti-CytokeratinMicroBeads.Cellsareperme
ABIlized,fixed,andincubatedwithAnti-CytokeratinMicroBeadsfordirectimmunomagneticlabelingofintracellularcytokeratin.ThemagneticallylabeledcellsarethenenrichedusingMSorLSColumns.
Downstreamapplications
Sincetheseparationprocedurepreservescellmorphology,carcinomacellscanquicklyandeasilybeidentifiedandenumeratedbyimmunocytochemistry,immunofluorescencemicroscopy,orflowcytometry.Enrichedcarcinomacellscanalsobefurtheranalyzedbymolecular
BIOLOGymethods.Forexample,singletumorcellsandtumorcellclustershavebeenenrichedforfurthercharacterization,e.g.byDNAstainingandimmunocytochemistry
3,4.
Columns
MSColumns.