Capacity:for2×109totalcellsOverview
TheCD34MultiSortKithasbeendevelopedforthepositiveselectionofCD34
+subpopulationsbysequentialsorting.CD34
+hematopoieticstemand
Progenitorcellscanbeisolatedfrom
- peripheralblood,
- bonemarrowaspirate,
- leukapheresisproduct,
- cordblood,
- differentiatedESandiPScellcultures1.
Details
Detailedseparationprocedure
UsingtheCD34MultiSortKit,inthefirststepCD34+cellsarepositivelyselectedafterlabelingwiththeCD34MultiSortMicroBeads.Subsequently,theMultiSortMicroBeadsareenzymaticallyreleasedfromtheCD34antibody.TheisolatedCD34+cellscanbemagneticallylabeledandsortedaccordingtoasecondantigen.ThekitcontainsCD34MultiSortMicroBeads,MultiSortReleaseReagent,andMultiSortStopReagent.
ControlstainingafterseparationrequiresamonoclonalCD34antibodyotherthanQBEND/10,e.g.cloneAC136
Downstreamapplications
TheCD34MultiSortKitisusedtoisolatespecificsubsetsofCD34+hematopoieticstemandprogenitorcells.CD34-selectedcellscanbefurthersortedaccordingtotheirexpressionof,e.g.,HLA-DR,CD43,CD45RA,CD61,CD71,CD90(Thy1),orCD133.
Columns
MS,LS,XS,orauto
MACS?Columns.