Content:25columnsOverview
LDColumnsarespeciallydesignedforstringentdepletionofunwantedcells.Evenweaklylabeledcellsareefficientlyretainedonthecolumn.
LDColumnscanbeusedwiththefollowingseparators:
- MidiMACS?Separator
- QuadroMACS?Separator
- VarioMACS?Separator,incombinationwithanLSColumnAdapter
- SuperMACS?IISeparator
- MultiMACSCell24SeparatorPlus,incombinationwiththeSingleColumnAdapter
Columncapacity
Forlymphoidcellsfromblood,bonemarrowortissue:
Upto108magneticallylabeledcellsfromupto5×108totalcells.
Forthecapacityforcellsfromothertissues,e.g.,neuraltissues,pleaserefertothedatasheetprovidedwiththeparticularMACSCellSeparationReagent.
ForusewiththeMultiMACSCell24SeparatorPlus:
Upto108magneticallylabeledcellsfromupto5×108totalcells.
Details
Backgroundinformation
ThematrixofLDColumnsiscomposedofferromagneticspheres,whicharecoveredwithacell-friendlycoating.WhenplacedinthemagneticfieldofaMACS
?Separator,thespheresamplifythemagneticfieldby10,000-fold,thusinducingahighgr
ADIentwithinthecolumn.ThisiscrucialforisolationofcellswhichareonlyminimallylabeledwithMACS
?MicroBeads,leavingenoughepitopesfreeforconcurrentantibodystaining.Thespacebetweenthespheresisseveraltimeslargerthanprimaryandmostculturedcells.Thisallowsthecellstofreelyflowthroughthecolumn.Magneticallylabeledcellsareheldins
USPensionwithinthecolumnanddonotactually“bind”thecolumnmatrix.Thissuspensionminimizesstressonthecellsandallowsforefficientsterilewashingbyavoidingcellaggregation.
LDColumnscanbeusedtoseparatemateriallessthan30μminsize.Thecolumnsareindividuallypackedinasterileway.