CSandDColumnsaresuitableforoptimaldepletionoflargecellnumbers.Byusingthedifferentflowresistorssuppliedwiththecolumns,theflowratecanbeadjusted,allowingefficientdepletionevenofcellsthatexpresstheseparation
Markerdimly.
CSColumnscanbeusedwiththeVario
MACS?,orSuperMACS?IISeparator.
DColumnsfitintotheSuperMACSIISeparator.
ThematrixofCSandDColumnsiscomposedofferromagneticfiberscoveredwithacell-friendlycoating.WhenplacedinthemagneticfieldofaMACS
?Separator,thefibersamplifythemagneticfield,thusinducingahighgr
ADIentwithinthecolumn.ThisiscrucialforisolationofcellswhichareonlyminimallylabeledwithMACS
?MicroBeads,leavingenoughepitopesfreeforconcurrentantibodystaining.Thespacebetweenthefibersisseveraltimeslargerthanprimaryandmostculturedcells.Thisallowsthecellstofreelyflowthroughthecolumn.Magneticallylabeledcellsareheldins
USPensionwithinthecolumnanddonotactually“bind”thecolumnmatrix.Thissuspensionminimizesstressonthecellsandallowsforefficientsterilewashingbyavoidingcellaggregation.
Thecolumnsareindividuallypackedinasterileway.