Immunogen | KLH-conjugated,syntheticpeptidecorrespondingtoaminoacids...QR(pY)SS...,inwhichpYcorrespondstophospho-tyrosineatresidue1069ofhumanepidermalgrowthfactorreceptor(EGFR) |
Host | Rabbit |
Specificity | phospho-EGFR(Tyr1069).Tyr1069correspondstothepositioninthehuman(isoforma)andmouseprecursorproteins.ThiscorrespondstoTyr1044(indicatedasTyr1045inseveralpublications)inthematurehumanandmouseprotein,andtoTyr1068intheratandpigprecursorprotein. |
SpeciesReactivity | |
SpeciesReactivityNote | Predictedtocrossreactwithbovine,canine,andporcinebasedonsequencehomology |
AntibodyType | PolyclonalAntibody |
EntrezGeneNumber | |
GeneSymbol | |
Modifications | |
PurificationMethod | Serum |
UniProtNumber | |
UniProtSummary | FUNCTION:SwissProt:P00533#Isoform2/truncatedisoformmayactasanantagoNIST. SIZE:1210aminoacids;134277Da SUBUNIT:BindsRIPK1.CBLinteractswiththeautophosphorylatedC-terminaltailoftheEGFreceptor.PartofacomplexwithERBB2andeitherPIK3C2AorPIK3C2B.TheautophosphorylatedforminteractswithPIK3C2B,maybeindirectly.InteractswithPELP1. SUBCELLULARLOCATION:Cellmembrane;Single-passtypeImembraneprotein.&Isoform2:Secreted. TISSUESPECIFICITY:Expressedinplacenta.Isoform2isalsoexpressedinovariancancers. PTM:PhosphorylationofSer-695ispartialandoccursonlyifThr-693isphosphorylated.&MonoubiquitinatedandpolyubiquitinateduponEGFstimulation;whichdoesnotaffecttyrosinekinaseactivityorsignalingcapacitybutmayplayaroleinlysosomaltargeting.Polyubiquitinlinkageismainlythrough"Lys-63",butlinkagethrough"Lys-48","Lys-11"and"Lys-29"alsooccur.DISEASE:SwissProt:P00533#DefectsinEGFRareassociatedwithlungcancer[MIM:211980]. SIMILARITY:SwissProt:P00533##Belongstotheproteinkinasesuperfamily.Tyrproteinkinasefamily.EGFreceptorsubfamily.&Contains1proteinkinasedomain. MISCELLANEOUS:BindingofEGFtothereceptorleadstodimerization,internalizationoftheEGF-receptorcomplex,inductionofthetyrosinekinaseactivity,stimulationofcellDNAsynthesis,andcellproliferation. |
MolecularWeight | 170-180kDa |