Immunogen | KLH-conjugatedlinearpeptidecorrespondingto9aminoacidsfromtheC-terminusofhumanHistoneH2Btype3-B.
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Concentration | PleaserefertotheCertificateofAnalysisforthelot-specificconcentration. |
Host | Rabbit |
Specificity | ThisrabbitpolyclonalantibodydetectsHistoneH2Binhumanandchicken.Ittargetsanepitopewithin9aminoacidsfromtheC-terminusregion.
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Isotype | IgG |
SpeciesReactivity | |
SpeciesReactivityNote | Human,Chicken.PredictedtoreactwithYeast,Xenopusbasedon100%sequencehomology.
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AntibodyType | PolyclonalAntibody |
EntrezGeneNumber | |
EntrezGeneSummary | Histonesarebasicnuclearproteinsthatareresponsibleforthenucleosomestructureofthechromosomalfiberineukaryotes.Nucleosomesconsistofapproximately146bpofDNAwrappedaroundahistoneoctamercomposedofpairsofeachofthefourcorehistones(H2A,H2B,H3,andH4).Thechromatinfiberisfurthercompactedthroughtheinteractionofalinkerhistone,H1,withtheDNAbetweenthenucleosomestoformhigherorderchromatinstructures.ThisgeneisintronlessandencodesamemberofthehistoneH2Bfamily.Transcriptsfromthisgenecontainapalindromicterminationelement. |
GeneSymbol | - GL105
- H2B
- H2B-GL105
- H2B.1
- H2B.q
- H2B/q
- H2BFQ
- MGC119802
- MGC119804
- MGC129733
- MGC129734
- OTTHUMP00000013920
|
PurificationMethod | ProteinAchromatography |
UniProtNumber | |
UniProtSummary | FUNCTION:SwissProt:Q8N257#Corecomponentofnucleosome.NucleosomeswrapandcompactDNAintochromatin,limitingDNAaccessibilitytothecellularmachinerieswhichrequireDNAasatemplate.Histonestherebyplayacentralroleintranscriptionregulation,DNArepair,DNAreplicationandchromosomalstability.DNAaccessibilityisregulatedviaacomplexsetofpost-translationalmodificationsofhistones,alsocalledhistonecode,andnucleosomeremodeling. SIZE:126aminoacids;13908Da SUBUNIT:ThenucleosomeisahistoneoctamercontainingtwomoleculeseachofH2A,H2B,H3andH4assembledinoneH3-H4heterotetramerandtwoH2A-H2Bheterodimers.Theoctamerwrapsapproximately147bpofDNA. SUBCELLULARLOCATION:Nucleus. PTM:MonoubiquitinationofLys-121bytheRNF20/40complexgivesaspecifictagforepigenetictranscriptionalactivationandisalsoprerequisiteforhistoneH3"Lys-4"and"Lys-79"methylation.ItalsofunctionscooperativelywiththeFACTdimertostimulateelongationbyRNApolymeraseII.&PhosphorylatedonSer-15bySTK4/MST1duringapoptosis;whichfacilitatesapoptoticchromatincondensation.AlsophosphorylatedonSer-15inresponsetoDNAdoublestrandbreaks(DSBs),andincorrelationwithsomatichypermutationandimmunoglobulinclass-switchrecombination. SIMILARITY:SwissProt:Q8N257##BelongstothehistoneH2Bfamily. |
MolecularWeight | ~17kDaobserved;13.91kDacalculated.Uncharacterizedbandsmaybeobservedinsomelysate(s). |