Immunogen | KLH-conjugatedphosphopeptidecontainingthepTEpYmotifofErk1andErk2.Clone:12D4 |
Clone | clone12D4 |
Concentration | PleaserefertotheCertificateofAnalysisforthelot-specificconcentration. |
Host | Mouse |
Specificity | Specificforphosphorylated(activated)Erk1andErk2.AntibodyrecognitionrequiresphosphorylationofboththethreonineandtyrosineresiduesofErk1/Erk2andwillnotinteractwiththenon-phosphorylatedformoftheproteins.DoesnotcrossreactwithactivatedSAPKinase1or2. |
Isotype | IgG1κ |
SpeciesReactivity | |
AntibodyType | MonoclonalAntibody |
EntrezGeneNumber | |
EntrezGeneSummary | TheproteinencodedbythisgeneisamemberoftheMAPkinasefamily.MAPkinases,alsoknownasextracellularsignal-regulatedkinases(ERKs),actinasignalingcascadethatregulatesvariouscellularprocessessuchasproliferation,differentiation,andcellcycleprogressioninresponsetoavarietyofextracellularsignals.Thiskinaseisactivatedbyupstreamkinases,resultinginitstranslocationtothenucleuswhereitphosphorylatesnucleartargets.Alternativelysplicedtranscriptvariantsencodingdifferentproteinisoformshavebeendescribed.... |
GeneSymbol | - MAPK3
- MGC20180
- ERT2
- HUMKER1A
- p44-MAPK
- PRKM3
- ERK1
- p44erk1
- p44mapk
- P44MAPK
- HS44KDAP
- p44-ERK1
- P44ERK1
- ERK-1
|
Modifications | |
PurificationMethod | Thiophilicadsoptionandsizeexclusionchromatography |
UniProtNumber | |
UniProtSummary | FUNCTION:SwissProt:P27361#Involvedinboththeinitiationandregulationofmeiosis,mitosis,andpostmitoticfunctionsindifferentiatedcellsbyphosphorylatinganumberoftranscriptionfactorssuchasELK-1.PhosphorylatesEIF4EBP1;requiredforinitiationoftranslation.Phosphorylatesmicrotubule-associatedprotein2(MAP2).PhosphorylatesSPZ1(Bysimilarity).Phosphorylatesheatshockfactorprotein4(HSF4). COFACTOR:Magnesium(Bysimilarity). SIZE:379aminoacids;43136Da SUBUNIT:InteractswithMORG1(Bysimilarity).BindstoHIV-1Nef.Thisinteractioninhibitsitskinaseactivity.InteractswithHSF4. DOMAIN:SwissProt:P27361TheTXYmotifcontainsthethreonineandtyrosineresidueswhosephosphorylationactivatestheMAPkinases. PTM:DuallyphosphorylatedonThr-202andTyr-204,whichactivatestheenzyme. SIMILARITY:Belongstotheproteinkinasesuperfamily.CMGCSer/Thrproteinkinasefamily.MAPkinasesubfamily.&Contains1proteinkinasedomain.... |
MolecularWeight | 42-44kDa |