Immunogen | Syntheticpeptide(C-KQLLHGEPNVS[pY]ICSRY)correspondingtoaminoacids203-219derivedfromaregionofthecatalyticdomain(betweensuBDomainVIIandVIII)oftheDrosophilaGSK3/shaggyenzymecoupledtoovalbumin. |
Epitope | SurroundingpTyr214(DrosophilaGSK-3),pTyr279(humanGSK3α),pTyr216(humanGSK3β) |
Clone | 5G-2F |
Concentration | PleaserefertotheCertificateofAnalysisforthelot-specificconcentration. |
Host | Mouse |
Specificity | RecognizesGSK-3phosphorylatedatthetargettyrosineresidue(humanGSK-3αpY279andGSK-3βpY216).The17-aminoacidimmunogenpeptidesequencediffersfromthecorrespondinghuman,mouse,andratsequencebyonlytwoaminoacidresidues. |
Isotype | IgG1κ |
SpeciesReactivity | |
SpeciesReactivityNote | Human,mouse,andrat.PredictedtoreactwithGSK-3orthologsfromdrosophilamelanogasterandmanyotherspeciesbasedonsequencehomologysurroundingthetargetphosphotyrosineresidue. |
AntibodyType | MonoclonalAntibody |
EntrezGeneNumber | |
EntrezGeneSummary | Glycogensynthasekinase3-alpha(GSK3A;EC{2.7.1.37})isamultifunctionalproteinserinekinase,homologoustoDrosophila"shaggy"(zeste-white3)andimplicatedinthecontrolofseveralregulatoryproteinsincludingglycogensynthase(seeGYS1,{138570})andtranscriptionfactors(e.g.,JUN,{165160}).ItalsoplaysaroleintheWNT({164820})andPI3K(seePIK3CG,{601232})signalingpathways(seereviewby{1:Alietal.(2001)}).[suppliedbyOMIM] |
GeneSymbol | |
Modifications | |
PurificationMethod | ProteinGChromatography |
UniProtNumber | |
UniProtSummary | FUNCTION:SwissProt:P49840#Implicatedinthehormonalcontrolofseveralregulatoryproteinsincludingglycogensynthase,MYBandthetranscriptionfactorJUN(Bysimilarity). SIZE:483aminoacids;50981Da SUBUNIT:Monomer(Bysimilarity). SIMILARITY:SwissProt:P49840##Belongstotheproteinkinasesuperfamily.CMGCSer/Thrproteinkinasefamily.GSK-3subfamily.&Contains1proteinkinasedomain. |
MolecularWeight | 51kDa(alpha)and46kDa(beta) |