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Millipore/05-1003 | Anti-phospho-Akt (Ser473) Antibody, clone 6F5/05-1003/100 µg
  • Millipore/05-1003 | Anti-phospho-Akt (Ser473) Antibody, clone 6F5/05-1003/100 µg

Millipore/05-1003 | Anti-phospho-Akt (Ser473) Antibody, clone 6F5/05-1003/100 µg

價(jià)格: ￥3948.00 市場(chǎng)價(jià): 6580.00
貨號(hào): 05-1003
品牌: Millipore
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    • Description
      CatalogueNumber05-1003
      DescriptionAnti-phospho-Akt(Ser473)Antibody,clone6F5
      AlternateNames
      • ProteinkinaseB
      • RAC-alphaserine/threonine-proteinkinase
      • murinethymomaviral(v-akt)oncogenehomolog-1
      • racproteinkinasealpha
      • v-aktmurinethymomaviraloncogenehomolog1
      BackgroundInformationAkt/PKBisaSer/ThrkinaseandamajorknowneffecterofthePI3Kinasepathway.ItisinvolvedinmultiplesignalingpathwaysthatrelatetomanyBIOLOGicalprocessesincludingglucosemetabolism,cellsurvival/apoptosis,cellcyclecontrol,angiogenesis,differentiation,andcellgrowthandproliferation.InmammalsthreeisoformsofAkt(Akt1/PKB?,Akt2/PKB?,andAkt3/PKB??)exists.Theyexhibitahighdegreeofhomology,butdifferslightlyinthelocalizationoftheirregulatoryphosphorylationsites.Akt?isthepredominantisoformthatisinmosttissuesandisthoughttohaveadominantroleingrowth,survival,embryonicdevelopment,andADIpocytedifferentiation.Akt2iscorrelatedwiththeregulationofglucosehomeostasisandisthepredominantisoformexpressedininsulin-responsivetissues.Akt3isabundantinbraintissue.EachAktisoformiscomposedofthreefunctionallydistinctregions:anN-terminalPleckstrinHomology(PH)domainthatprovidesalipid-bindingmoduletodirectAkttoPIP3atthecellmembranethatisnecessaryforitsactivation,acentralcatalyticdomaincontainingThr308,andaC-terminalhydrophobicmotifcontainingSer473.TheactivationofAktisdependentonadualregulatorymechanismthatrequiresbothitstranslocationtotheplasmamembraneanddualphosphorylationonThr308andSer473byPDK1andtheTORC2complex,respectively.
      ProductInformation
      FormatPurified
      PresentationPurifiedmousemonolconalIgG1κin0.1MTris-Glycine(pH7.4),150mMNaClwith0.05%NaN3
      StorageandShippingInformation
      StorageConditionsStablefor1yearat2-8oCfromdateofreceipt.
      Applications
      ApplicationAnti-phospho-Akt(Ser473)Antibody,clone6F5detectslevelofphospho-Akt(Ser473)&hasbeenpublished&validatedforuseinELISA,WB,FC,IF,IH.
      KeyApplications
      • ELISA
      • WesternBlotting
      • FlowCytometry
      • Immunofluorescence
      • Immunohistochemistry
      ApplicationNotesImmunofluroescenceAnalysis:
      EGF-treatedA431cellswerefixed,permeABIlized,andstainedwithAnti-phospho-Akt(Ser473),clone6F5(2μg/mL)(red).Cy3-conjugatedanti-mousewasusedfordetectionandPhalloidin-AlexaFluor?488(green)wasusedtostainactin.

      Immunohistochemistry:
      Anti-phospho-Akt(Ser473),clone6F5wasdilutedto1:200,IHC-SelectDetectionwithHRP-DAB.

      FlowCytometry:
      Anti-phospho-Akt(Ser473)wasusedata1:800dilutionforthisassay.
      BiologicalInformation
      ImmunogenPeptideencompassingandincludingphosphorylatedSer473ofhumanAkt1.
      EpitopePhosphorylatedSer473
      Clone6F5
      ConcentrationPleaserefertotheCertificateofAnalysisforthelot-specificconcentration.
      HostMouse
      SpecificityRecognizesAktonlywhenphosphorylatedonSer473.Thisantibodydoesnotcross-reactwithAkt1/PKBαwhenitisnotphosphorylatedonSer473.
      IsotypeIgG1κ
      SpeciesReactivity
      • Human
      • Mouse
      • Rat
      • Vertebrates
      SpeciesReactivityNoteHuman,mouse,andrattested.Expectedtoreactwithmostspeciesassequenceis100%homologouswithmostcommonvertebrate.
      AntibodyTypeMonoclonalAntibody
      EntrezGeneNumber
      EntrezGeneSummaryTheserine-threonineproteinkinaseencodedbytheAKT1geneiscatalyticallyinactiveinserum-starvedprimaryandimmortalizedfibroblasts.AKT1andtherelatedAKT2areactivatedbyplatelet-derivedgrowthfactor.Theactivationisrapidandspecific,anditisabrogatedbymutationsinthepleckstrinhomologydomainofAKT1.Itwasshownthattheactivationoccursthroughphosphatidylinositol3-kinase.InthedevelopingnervoussystemAKTisacriticalmediatorofgrowthfactor-inducedneuronalsurvival.Survivalfactorscansuppressapoptosisinatranscription-independentmannerbyactivatingtheserine/threoninekinaseAKT1,whichthenphosphorylatesandinactivatescomponentsoftheapoptoticmachinery.Multiplealternativelysplicedtranscriptvariantshavebeenfoundforthisgene.[providedbyRefSeq].
      GeneSymbol
      • AKT
      • C-AKT
      • EC2.7.11.1
      • MGC99656
      • MGC99656
      • PKB-ALPHA
      • PRKBA
      • RAC
      • RAC-ALPHA
      • RAC-PK-alpha
      Modifications
      • Phosphorylation
      PurificationMethodProteinGPurified
      UniProtNumber
      UniProtSummaryFUNCTION:Generalproteinkinasecapableofphosphorylatingseveralknownproteins.PhosphorylatesTBC1D4.Signalsdownstreamofphosphatidylinositol3-kinase(PI(3)K)tomediatetheeffectsofvariousgrowthfactorssuchasplatelet-derivedgrowthfactor(PDGF),epidermalgrowthfactor(EGF),insulinandinsulin-likegrowthfactorI(IGF-I).Playsaroleinglucosetransportbymediatinginsulin-inducedtranslocationoftheGLUT4glucosetransportertothecellsurface.MediatestheantiapoptoticeffectsofIGF-I.Mediatesinsulin-stimulatedproteinsynthesis,partlybyplayingaroleinbothinsulin-inducedphosphorylationof4E-BP1andininsulin-inducedactivationofp70S6kinase.Promotesglycogensynthesisbymediatingtheinsulin-inducedactivationofglycogensynthase.Ref.4Ref.8Ref.12Ref.13Ref.16Ref.20

      CatalyticactivityATP+aprotein=ADP+aphosphoprotein.Ref.4Ref.20Ref.1

      ENZYMEREGULATION:Threespecificsites,oneinthekinasedomain(Thr-308)andthetwootheronesintheC-terminalregulatoryregion(Ser-473andTyr-474),needtobephosphorylatedforitsfullactivation.Ref.8Ref.6

      SUBUNITSTRUCTURE:InteractswithAGAP2isoform2(PIKE-A)inthepresenceofguaninenucleotides.TheC-terminusinteractswithCCDC88A/GRDNandTHEM4.InteractswithAKTIP.Interacts(viaPHdomain)withMTCP1,TCL1AANDTCL1B.InteractswithCDKN1B;theinteractionphosphorylatesCDKN1Bpromoting14-3-3bindingandcell-cycleprogression.Ref.13Ref.16Ref.20Ref.9Ref.10Ref.11Ref.15Ref.17Ref.18Ref.19

      SUBCELLULARLOCATION:Cytoplasm.Nucleus.Cellmembrane.Note=Nucleusafteractivationbyintegrin-linkedproteinkinase1(ILK1).NucleartranslocationisenhancedbyinteractionwithTCL1A.Ref.11

      TISSUESPECIFICTY:Inallhumancelltypessofaranalyzed.Ref.4

      DOMAIN:BindingofthePHdomaintothephosphatidylinositol3-kinasealpha(PI(3)K)resultsinitstargetingtotheplasmamembrane.

      TheAGC-kinaseC-terminalmediatesinteractionwithTHEM4.

      PTM:PhosphorylationonThr-308,Ser-473andTyr-474isrequiredforfullactivity.Ser-473phosphorylationbytheRictor-mTorcomplexfavorsThr-308phosphorylationbyPDPK1.Ser-473phosphorylationisenhancedbyinteractionwithAGAP2isoform2(PIKE-A).Ser-473phosphorylationisenhancedinfocalcorticaldysplasiaswithTaylor-typeballooncells.

      SIMILARITY:Belongstotheproteinkinasesuperfamily.AGCSer/Thrproteinkinasefamily.RACsubfamily.

      Contains1AGC-kinaseC-terminaldomain.

      Contains1PHdomain.

      Contains1proteinkinasedomain.
      MolecularWeight60kDa
      PhysicochemicalInformation
      Dimensions
      MaterialsInformation
      MaterialsInformation
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