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Millipore/04-217 | Anti-phospho-c-Myc (Thr58/Ser62) Antibody, rabbit monoclonal/04-217/100 µL
  • Millipore/04-217 | Anti-phospho-c-Myc (Thr58/Ser62) Antibody, rabbit monoclonal/04-217/100 µL

Millipore/04-217 | Anti-phospho-c-Myc (Thr58/Ser62) Antibody, rabbit monoclonal/04-217/100 µL

價格: ¥3696.00 市場價: 6160.00

貨號: 04-217
品牌: Millipore
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    • Description
      CatalogueNumber04-217
      BrandFamilyUpstate
      TradeName
      • Upstate
      DescriptionAnti-phospho-c-Myc(Thr58/Ser62)Antibody,rabbitmonoclonal
      AlternateNames
      • Transcriptionfactorp64
      • avianmyelocytomatosisviraloncogenehomolog
      • mycproto-oncogeneprotein
      • v-mycavianmyelocytomatosisviraloncogenehomolog
      • v-mycmyelocytomatosisviraloncogenehomolog(avian)
      BackgroundInformationMycisamemberofthehelix-loop-helix-leucinezipper(bHLH-LZ)familyoftranscriptionfactors,whichdimerizeswithMaxtoactivatetranscriptionofsomegenesandrepressothers.AsMaxisessentialforMycactivity,theavailABIlityofMaxisaregulatorymechanismforMycactivity,andmembersoftheMadfamilyofproteinscompetewithMycforMax.
      ProductInformation
      FormatCultureSupernatant
      Control
      • Calyculin/Okadaicacid-tretedA431lysate
      Presentation100μLofrabbitmonoclonalIgGin60%storagebuffer(50mMTris-Glycine(pH7.4),0.15MNaCl,0.01%sodiumazideand0.05%BSA)and40%glycerol.
      StorageandShippingInformation
      StorageConditionsStablefor1yearat-20oCfromdateofreceipt.
      HandlingRecommendations:Uponfirstthaw,andpriortoremovingthecap,centrifugethevialandgentlymixthesolution.Aliquotintomicrocentrifugetubesandstoreat-20°C.Avoidrepeatedfreeze/thawcycles,whichmaydamageIgGandaffectproductperformance.Note:Variabilityinfreezertemperaturesbelow-20°Cmaycauseglycerolcontainingsolutionstobecomefrozenduringstorage.
      Applications
      ApplicationAnti-phospho-c-Myc(Thr58/Ser62)Antibodyisanantibodyagainstphospho-c-Myc(Thr58/Ser62)foruseinIF,IP&WB.
      KeyApplications
      • Immunofluorescence
      • Immunoprecipitation
      • WesternBlotting
      ApplicationNotesSuggesteddilutions:
      WesternBlot:1:10,000
      Immunocytochemistry:1:50-1:100
      Immunoprecipitation:1:100
      BIOLOGicalInformation
      ImmunogenSyntheticpeptidecorrespondingtosequencesurroundingandincludingphosphorylatedThr58andSer62ofhumanc-Myc.
      EpitopephosphorylatedThr58/Ser62
      HostRabbit
      Specificityc-MycwhenphosphorylatedonresiduesThr58/Ser62.
      IsotypeIgG
      SpeciesReactivity
      • Human
      • Mouse
      AntibodyTypeMonoclonalAntibody
      EntrezGeneNumber
      EntrezGeneSummaryTheproteinencodedbythisgeneisamultifunctional,nuclearphosphoproteinthatplaysaroleincellcycleprogression,apoptosisandcellulartransformation.Itfunctionsasatranscriptionfactorthatregulatestranscriptionofspecifictargetgenes.Mutations,overexpression,rearrangementandtranslocationofthisgenehavebeenassociatedwithavarietyofhematopoietictumors,leukemiasandlymphomas,includingBurkittlymphoma.Thereisevidencetoshowthatalternativetranslationinitiationsfromanupstream,in-framenon-AUG(CUG)andadownstreamAUGstartsiteresultintheproductionoftwoisoformswithdistinctN-termini.Thesynthesisofnon-AUGinitiatedproteinissuppressedinBurkitt"slymphomas,suggestingitsimportanceinthenormalfunctionofthisgene.
      GeneSymbol
      • c-Myc
      • C-Myc
      • MYC
      • C-MYC
      Modifications
      • Phosphorylation
      Non-ReactiveSpecies
      • Rat
      PurificationMethodUnpurified
      UniProtNumber
      UniProtSummaryFUNCTION:SwissProt:P01106#Participatesintheregulationofgenetranscription.BindsDNAbothinanon-specificmannerandalsospecificallytorecognizesthecoresequence5"-CAC[GA]TG-3".Seemstoactivatethetranscriptionofgrowth-relatedgenes.
      SIZE:439aminoacids;48804Da
      SUBUNIT:EfficientDNAbindingrequiresdimerizationwithanotherbHLHprotein.BindsDNAasaheterodimerwithMAX.InteractswithTAF1CandSPAG9.InteractswithPARP10.InteractswithJARID1AandJARID1B.
      SUBCELLULARLOCATION:Nucleus.
      PTM:PhosphorylatedbyPRKDC.
      DISEASE:SwissProt:P01106#OverexpressionofMYCisimplicatedintheetiologyofavarietyofhematopoietictumors.&AchromosomalaberrationinvolvingMYCmaybeacauseofaformofB-cellchroniclymphocyticleukemia.Translocationt(8;12)(q24;q22)withBTG1.
      SIMILARITY:SwissProt:P01106##Contains1basichelix-loop-helix(bHLH)domain.
      MolecularWeight57kDa
      PhysicochemicalInformation
      Dimensions
      MaterialsInformation
      MaterialsInformation
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