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Millipore/04-1002 | Anti-β-Catenin (NT) Antibody, clone E247, rabbit monoclonal/04-1002/100 µL
  • Millipore/04-1002 | Anti-β-Catenin (NT) Antibody, clone E247, rabbit monoclonal/04-1002/100 µL

Millipore/04-1002 | Anti-β-Catenin (NT) Antibody, clone E247, rabbit monoclonal/04-1002/100 µL

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貨號(hào): 04-1002
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    • Description
      CatalogueNumber04-1002
      DescriptionAnti-β-Catenin(NT)Antibody,cloneE247,rabbitmonoclonal
      AlternateNames
      • catenin(cadherin-associatedprotein),beta1(88kD)
      • catenin(cadherin-associatedprotein),beta1,88kDa
      • cateninbeta-1
      BackgroundInformationΒ-CateninlinksCadherincelladhesionproteinstotheCytoskeletonandhasrecentlybeenimplicatedintheWnt/Winglesssignalingpathwayandintheprogressionofneoplasia.β-CateninassociateswiththetumorsuppressorAdenomatousPolypopsisColi(APC)andfunctionsasaco-activatoroftheTCFtranscriptionfactorfamily.ItisregulatedbyGlycogenSynthaseKinase(GSK)3b,whichphosphorylatesandtargetsβ-Cateninforubiquitin-mediatedproteolysis.StABIlizationofβ-Catenin,eitherbyWnt-mediatedinhibitionofGSK3borbymutation(asfoundinnumerouscolorectaltumorsandmelanomacelllines),resultsinactivationofTCFresponsivegeneswhicharethoughttoplaykeyrolesindevelopmentandcancerprogression.δ-Catenin(NPRAP/Neurojungin),amemberofthep120Cateninsubfamily,isastructuralproteininvolvedinneuronaldevelopment.ItinteractswithActin,CortactinandSrcKinasestopromoteneuriteelongationorbranching,dependinguponitsphosphorylationstate.
      ProductInformation
      FormatUnpurified
      Control
      • A431celllysates
      PresentationRabbitmonoclonalIgGinbuffercontaining50mMTris-Glycine(pH7.4),0.15MNaClcontaining40%Glycerol,0.01%sodiumazideand0.05%BSA.
      StorageandShippingInformation
      StorageConditionsStablefor1yearat-20oCfromdateofreceipt.
      HandlingRecommendations:Uponreceipt,andpriortoremovingthecap,centrifugethevialandgentlymixthesolution.Aliquotintomicrocentrifugetubesandstoreat-20°C.Avoidrepeatedfreeze/thawcycles,whichmaydamageIgGandaffectproductperformance.Note:Variabilityinfreezertemperaturesbelow-20°Cmaycauseglycerolcontainingsolutionstobecomefrozenduringstorage.
      Applications
      ApplicationPleasenotethatthisproductwillnotbeavailableforsaleafterMarch15,2015.Pleaseselectoneoftheotherantibodiesagainstthistarget.Anti-β-Catenin(N-term)Antibody,cloneE247,Rabbitisanantibodyagainstβ-Catenin(N-term)foruseinIH,WB,IC&IP.
      KeyApplications
      • Immunohistochemistry
      • WesternBlotting
      • Immunocytochemistry
      • Immunoprecipitation
      ApplicationNotesImmunohistochemistryAnalysis:1:500dilutionfromapreviouslotdetectedβ-catenininhumancolonadenocarcinoma.

      ImmunocytochemistryAnalysis:1:250dilutionfromapreviouslotdetectedβ-catenininA431celllysate.
      BIOLOGicalInformation
      ImmunogenSyntheticpeptidecorrespondingtoresiduesneartheN-terminusofhumanβ-catenin.
      EpitopeN-terminus
      CloneE247
      HostRabbit
      SpecificityThisantibodyrecognizesβ-cateninatandaroundtheN-terminus.
      IsotypeIgG
      SpeciesReactivity
      • Human
      • Mouse
      AntibodyTypeMonoclonalAntibody
      EntrezGeneNumber
      EntrezGeneSummaryBeta-cateninisanadherensjunctionprotein.Adherensjunctions(AJs;alsocalledthezonulaadherens)arecriticalfortheestablishmentandmaintenanceofepitheliallayers,suchasthoseliningorgansurfaces.AJsmediateadhesionbetweencells,communicateasignalthatneighboringcellsarepresent,andanchortheactincytoskeleton.Inservingtheseroles,AJsregulatenormalcellgrowthandbehavior.Atseveralstagesofembryogenesis,woundhealing,andtumorcellmetastasis,cellsformandleaveepithelia.Thisprocess,whichinvolvesthedisruptionandreestablishmentofepithelialcell-cellcontacts,mayberegulatedbythedisassemblyandassemblyofAJs.AJsmayalsofunctioninthetransmissionofthe"contactinhibition"signal,whichinstructscellstostopdividingonceanepithelialsheetiscomplete.[suppliedbyOMIM].

      GeneSymbol
      • Beta-catenin
      • CTNNB
      • DKFZp686D02253
      • FLJ25606
      • FLJ37923
      • OTTHUMP00000165222
      • OTTHUMP00000165223
      PurificationMethodUnpurified
      UniProtNumber
      UniProtSummaryFUNCTION:InvolvedintheregulationofcelladhesionandinsignaltransductionthroughtheWntpathway.
      SUBUNITSTRUCTURE:Twoseparatepoolsarefoundinthecytoplasm:oneisPSEN1/cadherin/catenincomplexwhichanchorstotheactincytoskeleton.TheotherpoolispartofalargecomplexcontainingAXIN1,AXIN2,APC,CSNK1A1andGSK3BthatpromotesphosphorylationonN-terminalSerandThrresiduesandubiquitinationofCTNNB1viaBTRCanditssubsequentdegradationbytheproteasome.Wnt-dependentactivationofDVLantagonizestheactionofGSK3B.WhenGSK3Bactivityisinhibitedthecomplexdissociates,CTNNB1isdephosphorylatedandisnolongertargetedfordestruction.Thestabilizedproteintranslocatestothenucleus,whereitbindsTCF/LEF-1familymembers,TBP,BCL9andpossIBLyalsoRUVBL1andCHD8.BindsCTNNBIPandEP300.CTNNB1formsaternarycomplexwithLEF1andEP300thatisdisruptedbyCTNNBIP1bindingBysimilarity.InteractswithTAX1BP3(viathePDZdomain);thisinteractioninhibitsthetranscriptionalactivityofCTNNB1Bysimilarity.InteractswithAJAP1,BAIAP1,CARM1,CTNNA3,CXADRandPCDH11Y.BindsSLC9A3R1.InteractswithGLIS2andMUC1.InteractswithSLC30A9.InteractswithXIRP1Bysimilarity.InteractswithPTPRU(viathecytoplasmicjuxtamembranedomain).
      SUBCELLULARLOCATION:Cytoplasm.Nucleus.Cytoplasm?cytoskeleton.Note=Cytoplasmicwhenitisunstabilized(highlevelofphosphorylation)orboundtoCDH1.Translocatestothenucleuswhenitisstabilized(lowlevelofphosphorylation).InteractionwithGLIS2andMUC1promotesnucleartranslocation.
      TISSUESPECIFICITY:Expressedinseveralhairfolliclecelltypes:basalandperipheralmatrixcells,andcellsoftheouterandinnerrootsheats.Expressedincolon.
      PTM:PhosphorylationbyGSK3BrequirespriorphosphorylationofSer-45byanotherkinase.PhosphorylationproceedsthenfromThr-41toSer-37andSer-33.
      EGFstimulatestyrosinephosphorylation.PhosphorylationonTyr-654decreasesCDH1bindingandenhancesTBPbinding.
      UbiquitinatedbyaE3ubiquitinligasecomplexcontainingUBE2D1,SIAH1,CACYBP/SIP,SKP1A,APCandTBL1XProbable.Itsubiquitinationleadstoitssubsequentproteasomaldegradation.
      DISEASE:ActivatingmutationsinCTNNB1haveoncogenicactivityresultingintumordevelopment.Somaticmutationsarefoundinvarioustumortypes,includingcoloncancers,ovarianandprostatecarcinomas,hepatoblastoma(HB),hepatocellularcarcinoma(HCC).HBsaremalignantembryonaltumorsmainlyaffectingyoungchildreninthefirstthreeyearsoflife.
      DefectsinCTNNB1areacauseofpilomatrixoma(PTR)[MIM:132600];acommonbenignskintumor.
      DefectsinCTNNB1areacauseofmedulloblastoma(MDB)[MIM:155255].MDBisamalignant,invasiveembryonaltumorofthecerebellumwithapreferentialmanifestationinchildren.
      AchromosomalrearrangementinvolvingCTNNB1maybeacauseofsalivaryglandpleiomorphicadenomas(PA)[181030].Pleiomorphicadenomasarethemostcommonbenignepithelialtumorsofthesalivarygland.Translocationt(3;8)(p21;q12)withPLAG1.
      SIMILARITY:Belongstothebeta-cateninfamily.
      Contains12ARMrepeats.
      MolecularWeight~92kDa
      PhysicochemicalInformation
      Dimensions
      MaterialsInformation
      MaterialsInformation
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