當(dāng)前位置 ： NEB >>> NEB/HiFi Taq DNA Ligase/50 reactions/M0647S
NEB/HiFi Taq DNA Ligase/50 reactions/M0647S
  • NEB/HiFi Taq DNA Ligase/50 reactions/M0647S

NEB/HiFi Taq DNA Ligase/50 reactions/M0647S

價(jià)格: 試用 市場(chǎng)價(jià): 0.00
貨號(hào): M0647S
品牌: NEB
規(guī)格
數(shù)量
庫(kù)存(0)
特別 提示
代購(gòu)產(chǎn)品：無(wú)質(zhì)量問(wèn)題不接受退換貨，下單前請(qǐng)仔細(xì)核對(duì)信息。
下單后請(qǐng)及時(shí)聯(lián)系客服核對(duì)商品價(jià)格，訂單生效后再付款。
資深產(chǎn)品顧問(wèn)
咨詢顧問(wèn)

全國(guó)免費(fèi)服務(wù)熱線

4000-520-616

  • 自營(yíng)商城 一站式服務(wù)
  • 廠家直采 剔除溢價(jià)
  • 品質(zhì)甄選 正品保證
  • 嚴(yán)控流程 只做188精品
  • 極速物流 如約送貨
  • 詳情
  • 使用說(shuō)明
  • 常見(jiàn)問(wèn)題
    • ArticleAn optimized blend of a thermostable DNA Ligase and a proprietary additive, HiFi Taq DNA Ligase efficiently seals nicks in DNA with unmatched high fidelity. The formation of a phosphodiester bond between juxtaposed 5´ phosphate and 3´ hydroxyl termini of two adjacent oligonucleotides that are hybridized to a complementary target DNA is enhanced in the improved reaction buffer and mismatch ligation is dramatically reduced (1). The improved formulation allows higher resolution discrimination between ligation donors and acceptors, enabling precise detection of SNPs and other allele variants. HiFi Taq DNA Ligase is active at elevated temperatures (37–75°C) (2,3).Please note that HiFi Taq DNA Ligase is intended for use in molecular diagnostics applications that depend on high fidelity nick ligation. It is not a substitute for T4 DNA ligase and is not suitable for cloning applications or adapter ligation/NGS library prep.Figure 1: HiFi Taq DNA Ligase displays increased fidelity
      (A) Schematic of multiplexed substrate pools. Each substrate pool contained a single splint with a defined NN at the ligation junction (e.g., AA, AC, AG…) along with all four upstream probes and all four FAM-labeled downstream probes. Each probe that encodes the base at the ligation junction is of unique length allowing for separation and analysis by capillary electrophoresis. A total of 16 substrate pools were prepared, one for each unique splint. (B) Comparison of the ligation fidelity of Ampligase (Epicentre), Taq DNA Ligase and HiFi Taq DNA Ligase. Fidelity measurements were performed using 1 μl of ligase in a 50 μl reaction mixture in the supplied buffers at 1X concentration. Reactions were incubated 30 min at 55°C, using multiplexed substrate pools as outlined in (A). Rows represent a single template sequence, while columns indicate a particular ligation product resulting from a specific pair of probes ligating with the indicated bases at the ligation junction. A dot indicates detection of a product (see legend above). The diagonal from the top left to the bottom right represents Watson-Crick ligation products; all other spaces indicate mismatch ligation products. While Taq DNA Ligase and Ampligase perform similarly under these conditions, with a range of mismatch products detectable, HiFi Taq DNA Ligase shows dramatically fewer mismatch products while maintaining high yields (image adapted from Reference 1).
      Figure 2: HiFi Taq DNA Ligase exhibits increased discrimination at both sides of the ligation junction
      Oligonucleotide probes targeting a 35 bp region of the λ integrase gene were incubated with 16.7 fmol of λ genomic DNA. Ligation products formed were detected by qPCR using SYBR® Green. HiFi Taq DNA Ligase (NEB #M0647) displays increased fidelity over Taq DNA Ligase (NEB #M0208) and Ampligase, showing a larger ΔCt value between probes fully complementary to the target sequence and those with a mismatched base pair at the ligation junction.
      Figure 3: HiFi Taq DNA Ligase exhibits increased thermostability
      HiFi Taq DNA Ligase exhibits increased thermostability
      HiFi Taq DNA Ligase and Ampligase® (1 µl enzyme in a 50 µl reaction) were cycled (80°C for 90 seconds/94°C for 10seconds) up to 100times in their respective 1X reaction buffer. Ligase activity was assayed using a FAM-labeled nicked dsDNA substrate detected by capillary electrophoresis.
      Figure 4: HiFi Taq DNA Ligase displays 2-fold reduction in mismatch ligation as compared to Ampligase
      HiFi Taq DNA Ligase displays 2-fold reduction in mismatch ligation as compared to Ampligase
      The C:G Watson-Crick base pair between the upstream probe 3´-terminal base and the complementary strand makes this a particularly difficult junction for a ligase to discriminate against mismatch ligation products (1).

      Product Source

      Purified from an E. coli strain that carries the cloned ligase gene from a hyperthermophilic organism.
      This product is related to the following categories:
      DNA Ligases Products
      This product can be used in the following applications:
      Non-Cloning Ligation,
      DNA Ligation
    售后保障
    螞蟻淘生物188，秉承螞蟻淘一貫的嚴(yán)謹(jǐn)態(tài)度，由螞蟻淘公司專業(yè)人員負(fù)責(zé)品控、采購(gòu)、物流、銷售、售后，保障正品優(yōu)質(zhì)。以“快速好省，為科研提供好產(chǎn)品、好價(jià)格”為理念，直接鏈接原廠家，從全國(guó)各地原制造商嚴(yán)格挑選188款科研精品，剔除品牌溢價(jià)，188生物新電商，把好的產(chǎn)品帶給科研！? 力求給你最優(yōu)質(zhì)的商品。
  • Q：生物188產(chǎn)品正品保障嗎？
    A：生物188質(zhì)量把控人員具有十年的從業(yè)經(jīng)驗(yàn)，在業(yè)界享有良好的口碑；自營(yíng)商城，直接從廠家采購(gòu)， 自己的團(tuán)隊(duì)負(fù)責(zé)國(guó)際物流和清關(guān)，中間沒(méi)有第三方，所有流程嚴(yán)格把控，100%保證正品，假一罰十。

    Q：下單后可以修改訂單嗎？
    A：下單后的商品付款之前可以修改；訂單付款成功，需要聯(lián)系我們客服進(jìn)行修改；客服電話：4000-520-616

    Q：可以開(kāi)發(fā)票嗎？
    A：本網(wǎng)站所售商品都是正規(guī)清關(guān)，均開(kāi)具16%正規(guī)發(fā)票，發(fā)票金額含配送費(fèi)金額，另有說(shuō)明的除外。

    Q：商品幾天可以發(fā)貨？
    A：生物188商品，全部現(xiàn)貨銷售，付款后即可發(fā)貨,一般一周內(nèi)送達(dá)！

    Q：如何聯(lián)系商家？
    A：有任何問(wèn)題夠可以電話咨詢我們，全國(guó)24小時(shí)免費(fèi)服務(wù)熱線：4000-520-616 或聯(lián)系我們的在線客服QQ：1570468124

    Q：收到的商品少了/發(fā)錯(cuò)了怎么辦？
    A：同個(gè)訂單購(gòu)買多個(gè)商品可能會(huì)分為一個(gè)以上包裹發(fā)出，可能不會(huì)同時(shí)送達(dá)，建議查看訂單詳情是否是 部分發(fā)貨狀態(tài)；如未收到，可聯(lián)系在線客服或者致電4000-520-616。

    Q：退換貨/維修需要多長(zhǎng)時(shí)間？
    A：一般情況下，退貨處理周期為客戶收到產(chǎn)品一個(gè)月內(nèi)（以快遞公司顯示簽收時(shí)間為準(zhǔn)），包裝規(guī)格、 數(shù)量、品種不符，外觀毀損、短缺或缺陷，請(qǐng)?jiān)谑盏截?4小時(shí)內(nèi)申請(qǐng)退換貨；特殊商品以合同條款為準(zhǔn)。

何為188

極簡(jiǎn)而嚴(yán)謹(jǐn)，我們僅銷售188款生物醫(yī)學(xué)科研用品，款款都是爆款；因?yàn)樯偎跃劢?，聚焦甄選每一款產(chǎn)品，聚焦服務(wù)每一位客戶!

關(guān)注我們 :

點(diǎn)擊QQ聯(lián)系我們
生物188微信

關(guān)注188微信公眾號(hào)

獲取最新優(yōu)惠活動(dòng)通知