An affinity matrix for the small-scale isolation of target proteins fused to a chitin binding domain (CBD) (1). Chitin beads have been prepared having a magnetite core. This permits the magnetic isolation of CBD-fusion proteins from cell culture supernatants; after which, the matrix can be regenerated without loss of binding capacity. Immobilized fusion proteins can be used in subsequent experiments to capture target proteins from crude cell lysates that interact with the immobilized fusion protein.IMPACT Citations
For larger volume requirements, customized and bulk packaging is available by purchasing through the OEM/Bulks department at NEB. Please contactcustom@neb.comfor further information.
- This product is related to the following categories:
- Chitin Purification (CBD-tag),
- Affinity Purification,
- Magnetic Beads and Racks,
Protein Purification
- This product can be used in the following applications:
- Small RNA Detection and Isolation,
- Affinity Purification & Expression Tags,
- Protein Purification,
- High-throughput cloning and automation solutions,
IMPACT Affinity Tag
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